mLife · Volume 3 · Issue 3
于2024年9月30日正式出版
好文速览
扫码阅读本期原文
https://onlinelibrary.wiley.com/toc/2770100x/2024/3/3
第三卷Issue 3共计12篇文章，包括3篇Reviews，1篇Research Highlight，1篇Opinion，6篇Original Research，1篇Correspondence。作者分别来自中国、新加坡、加拿大、美国、法国等国家。
Reviews
▶Heme homeostasis and its regulation by hemoproteins in bacteria
Yingxi Li, Sirui Han, Haichun Gao
https://doi.org/10.1002/mlf2.12120
浙江大学生命科学学院微生物研究所高海春教授团队的文章综述了控制血红素稳态至关重要的生物过程的分子机制，包括合成、获取、利用、降解、运输和外流及其调节，同时涵盖了与血红素稳态相关的血红素蛋白及其系统的生理生化和结构特征的最新研究结果。
▶Staphylococcus aureusSOS response: Activation, impact, and drug targets
Kaiying Cheng, Yukang Sun, Huan Yu, Yingxuan Hu, Yini He, and Yuanyuan Shen
https://doi.org/10.1002/mlf2.12137
杭州师范大学程凯莹教授团队的综述文章全面总结了金黄色葡萄球菌SOS反应的激活和效应过程，以及该通路关键蛋白的功能和结构，提出了针对性治疗策略和展望。
▶Oral microbiome in human health and diseases
Siqi Tian, Tao Ding, Hui Li
https://doi.org/10.1002/mlf2.12136
The authors systematically review the relationship between oral microbiome dynamics and human diseases.
Research Highlight
▶Efficient, compact, and versatile: Type I-F2 CRISPR-Cas system
Shengsheng Ma, Senfeng Zhang, Kunming Liu, Tao Hu, Chunyi Hu
https://doi.org/10.1002/mlf2.12145
This Research Highlight is based on a study recently published in Nature Communications by Guo et al., which presents a milestone in genome editing and transcriptional regulation within human cells, leveraging the smallest Type I system known to date, the I-F2 subtype.
Opinion
▶Ectomycorrhizal fungi: Potential guardians of terrestrial ecosystems
Wenchen Song
https://doi.org/10.1002/mlf2.12127
中央民族大学宋文琛助理研究员的观点性文章提出了四个关于使用外生菌根真菌来应对人为活动引起的全球变化的建议。与其他内生菌根真菌如丛枝菌根真菌相比，外生菌根真菌更有可能成为"陆地生态系统的守护者"。
Original Research
▶Zinc finger 4 negatively controls the transcriptional activator Fzf1 inSaccharomyces cerevisiae
Ying Du, Chaoqun Ma, Stanley A. Moore, Wei Xiao
https://doi.org/10.1002/mlf2.12141
加拿大萨斯喀彻温大学萧伟教授团队基于锌指蛋白Fzf1开展了有关其作为转录因子的功能探究实验，发现其作为广谱转录因子可以被相对应的药物激活并诱导下游多个抗药胁迫相关蛋白基因的表达，且其第四锌指区域可以抑制自身的激活。
▶Catalytically active prokaryotic Argonautes employ phospholipase D family proteins to strengthen immunity against different genetic invaders
Feiyue Cheng, Aici Wu, Zhihua Li, Jing Xu, Xifeng Cao, Haiying Yu, Zhenquan Liu, Rui Wang, Wenyuan Han, Hua Xiang, Ming Li
https://doi.org/10.1002/mlf2.12138
中国科学院微生物研究所研究团队发现具有催化活性的原核Argonaute（pAgo）可以利用偶联的磷脂酶D（PLD）共同抵御不同外源DNA，而且PLD增强了pAgo介导的对单链DNA病毒的免疫能力；另外，研究团队进一步发现这类pAgo可以提高大肠杆菌的基因编辑效率。该研究凸显了高度多样的pAgo通过偶联其他效应蛋白共同抵御外源遗传元件的多样机制，并为基因编辑工具开发提供了新的元件。
▶Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing
Olivier Pereira, Wei Qin, Pierre E. Galand, Didier Debroas, Raphael Lami, Corentin Hochart, Yangkai Zhou, Jin Zhouand Chuanlun Zhang
https://doi.org/10.1002/mlf2.12144
The authors demonstrate that quorum sensing (QS) orchestrates nitrogen and carbon metabolism, including the exchange of organic metabolites between ammonia-oxidizing archaea (AOA) and surrounding heterotrophic bacteria, which has been previously overlooked in marine AOA research.
▶The phytopathogenXanthomonas campestrissenses and effluxes salicylic acid via a sensor HepR and an RND family efflux pump to promote virulence in host plants
Kai Song, Ruifang Li, Ying Cui, Bo Chen, Lian Zhou, Wenying Han, Bo-Le Jiang, Ya-Wen He
https://doi.org/10.1002/mlf2.12140
上海交通大学何亚文教授团队发现野油菜黄单胞菌XC1侵染寄主植物后诱导植物侵染部位大量产生SA。为了拮抗SA，XC1诱导表达一个SA感应蛋白HepR；HepR感应SA后, 启动一个RND-家族外排泵编码基因簇hepABCD的表达，外排SA，同时改变胞内pH和群体感应等生理功能，提高其致病性。
▶Discovery of β-nitrostyrene derivatives as potential quorum sensing inhibitors for biofilm inhibition and antivirulence factor therapeutics againstSerratia marcescens
Jiang Wang, Jingyi Yang, Pradeepraj Durairaj, Wei Wang, Dongyan Wei, Shi Tang, Haiqing Liu, Dayong Wang, Ai-Qun Jia
https://doi.org/10.1002/mlf2.12135
海南省人民医院贾爱群教授团队与海南大学药学院王大勇教授针对耐药病原菌粘质沙雷氏菌（S. marcescens）引起的感染治疗困难的问题，设计并合成了33个1-硝基-2-苯基乙烯(NPe)衍生物，并评估了其对S. marcescens的群体感应（QS）抑制活性，发现其中衍生物m-NPe对S. marcescensNJ01具有良好的生物被膜抑制/破坏作用和毒力因子抑制作用。
▶EmbB and EmbC reaulate the sensitivity ofMvcobacterium abscessusto echinomycin
Jing He, Yamin Gao, Jingyun Wang, H. M. Adnan Hameed, Shuai Wang, Cuiting Fang, Xirong Tian, Jingran Zhang, Xingli Han, Yanan Ju, Yaoju Tan, Junying Ma, Jianhua Ju, Jinxing Hu, Jianxiong Liu, Tianyu Zhang
https://doi.org/10.1002/mlf2.12139
中国科学院广州生物医药与健康研究院张天宇研究员团队与广州市胸科医院胡锦兴教授团队通过检测棘霉素（echinomycin，ECH）对脓肿分枝杆菌（Mab）的活性，发现ECH对Mab尤其是embC基因缺失的菌株具有极强的抗菌活性，EmbB和EmbC可以通过改变细胞壁的通透性影响Mab对ECH的敏感性。
Correspondence
▶Perchlorate-tolerant bacterial strains isolated from the Mars-analog Qaidam Basin soils exposed to Earth's near space
Li Liu, Mengling Kang, Zhe Wang, Jianxun Shen, Yongxin Pan, Wei Lin
https://doi.org/10.1002/mlf2.12142
中国科学院地质与地球物理研究所林巍研究员团队利用高空气球将柴达木盆地的土壤样品带到临近空间35公里高度开展暴露实验，该区域的综合环境条件可以类比现代火星表面环境。该研究从暴露后土壤中成功分离培养了具有较高的高氯酸盐耐受能力的四株细菌。
